KOD Hot Start DNA Polymerase* is a premixed complex of KOD DNA Polymerase and two monoclonal antibodies that inhibit the DNA polymerase and 3'→5' exonuclease activities at ambient temperatures (Mizuguchi 1999). 0
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With the KOD I got the correct clone in the first run already. No other patents rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. KOD Hot Start combines the high fidelity, fast extension speed, and outstanding processivity of KOD with the high specificity of an antibody-mediated hot start. KOD -Plus- is based on DNA polymerase from the hyperthermophilic Archaeon Thermococcus kodakaraensis KOD1(1)(2) KOD -Plus- exhibits excellent high PCR fidelity and efficiency. (See Table I for a comparison of Agilent hot start PCR enzymes.) 0000004736 00000 n
KOD Xtreme™ Hot Start DNA Polymerase is an optimised PCR enzyme for the amplification of long or GC-rich DNA templates, including mouse tail-tips, plant tissues and synthetic biology applications. DreamTaq Hot Start offers higher sensitivity, specificity, and yields compared to conventional hot start Taq DNA polymerase. Denaturation: KOD Xtreme Hot Start DNA Polymerase quickly and accurately amplifies genomic and phage/plasmid DNA targets up to 24 and 40 kbp, respectively. Further I got a stronger PCR- band, respectively more PCR-product with virtually no background than with our conventional polymerase. 0000000016 00000 n
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KOD Hot Start DNA Polymerase is a premixed complex of KOD DNA Polymerase and two monoclonal antibodies that inhibit the DNA polymerase and 3´â 5´ exonuclease activities at ambient temperatures. 0000011943 00000 n
The hyperthermophilic archaea Thermococcus kodakaraensis KOD1 (previously Pyrococcus kodakaraensis KOD1) (Fig. .. 0000035509 00000 n
D4001, D4005, D4006, D4101 Article Title: Ad 2.0: a novel recombineering platform for high-throughput generation of tailored adenoviruses Article Snippet: ..PCR For all PCRs high-fidelity KOD Hot Start DNA Polymerase (Novagen, Darmstadt) was used except for amplification of the BAC-backbone, which was performed with the PRECISOR polymerase (Biocat, Heidelberg). 0000012515 00000 n
(1997) Appl. In addition, optimized buffer compositions, convenient master mixes and cycling parameters provide additional ease of use and data reproducibility.KOD Hot Start DNA Polymerase* is a premixed complex of KOD DNA Polymerase and two monoclonal antibodies that inhibit the DNA polymerase and 3′→5′ exonuclease activities at ambient temperatures (Mizuguchi 1999). 132 bp, 251 bp, 1,005 bp, and 3.9 kb fragments were amplified from 50 ng of human genomic DNA in 50 μL reactions using Platinum II Taq Hot-Start DNA Polymerase or other hot-start DNA polymerases: (A) NEB OneTaq Hot Start DNA Polymerase, (B) Qiagen Fast Cycling PCR Kit, (C) … 0000008210 00000 n
Q5U contains a mutation in the uracil-binding pocket that enables the ability to read and amplify templates containing uracil and inosine bases. 0000003282 00000 n
Hot Start Taq DNA Polymerase is a mixture of Taq DNA Polymerase and an aptamer-based inhibitor. Hot start PCR is a modified form of conventional polymerase chain reaction (PCR) that reduces the presence of undesired products and primer dimersdue to non-specific DNA amplification at room (or colder) temperatures. Further information on purchasing licenses may be obtained by contacting the Director … 0000002561 00000 n
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KOD Xtreme™ Hot Start DNA Polymerase Ultra high fidelity dnapolymerase designed for the most challenging PCR applications including: crude sample PCR, amplification of long strand and highly GC-rich DNA templates. <<11a0da7e57cc8940b64f4a7dce07ee9a>]>>
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", "Excellent product that can be run by dummies due to its simple and clear instructions. 1631 52
Platinum II Taq Hot-Start DNA Polymerase enables cycling of shorter and longer amplicons together. Latest: KNAUER is expanding its business activities into the field of lipid nanoparticle... Catch highlights from the Virtual Biopharmaceutical Summit 2020, From horses to humans: Elucidating how bones heal after a fracture, EUROIMMUN launches novel ELISA for detection of SARS-CoV-2 antigen in acutely infected patients, Docking station enables a consistent sample tube (De)capping action, CAR T: Dual-targeted cell therapy to prevent relapsed multiple myeloma: Your questions answered, PCR/microplate sealing and peeling for high-throughput labs. 0000018915 00000 n
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The intrinsic high processivity of the enzyme results in significant improvement in yield, speed and sensitivity when compared with wild-type B-family DNA polymerases. 0000058035 00000 n
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